hydrophobic amino acid meaning in English
疏水氨基酸
Examples
- The deduced amino acid sequence of rbcl and rbcs includes 13 . 14 % acidic amino acid and 14 . 51 % aliphatic amino acid , the hydrophobic amino acid is 42 . 16 %
14的酸性氨基酸， 14 51脂肪族氨基酸，疏水性氨基酸的比例为42 16 。 - A newly made amino acid chain folds into a distinctive shape depending on the positioning of hydrophobic amino acids , which like to cluster together away from the cell ' s watery cytoplasm , leaving hydrophiles to form the protein ' s surface
刚合成好的胺基酸长链，会根据疏水性胺基酸的分布位置，摺叠成蛋白质的独特形状：疏水性胺基酸彼此向内聚拢，避免接触到富含水的细胞质环境；亲水性胺基酸则向外翻出，构成蛋白质的表面。 - Yuanbai was composed of 1 818 bp with a nucleotide orf ( 1 581 bp ) , which encoded a protein of 526 aa with molecular weight of 62 . 2 kda and pi of 8 . 96 . strongly basic ( + ) amino acids , strongly acidic ( - ) amino acids , hydrophobic amino acids and polar amino acids of the protein were 13 . 12 % , 11 . 59 % , 36 . 69 % and 23 . 19 % respectively
( 5 )将bccyp86材下i和天客cyps石初下基因转化到大肠杆菌blzi中诱导表达，发现t - 28 、 1mmol ? l ’ ，的iptg 、诱导20h的条件下， pgex一bccyp86叼fl 、 pgex .尺刃了尸召‘初下两个重组质粒均有约80kda的蛋白质特征条带出现。 - Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e . coli bl21 ( de3 ) . no squalene synthase polypeptide of expected molecular mass was observed in e . coli containing the putative full - length squalene synthase cdna , however , overexpression in e . coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全长的鲨烯合酶cdna的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达，而c末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。 - Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ) , which encoded a protein consisting of 524 aa with molecular weight of 62 . 2 kda and pi of 8 . 96 . strongly basic ( + ) amino acids , strongly acidic ( - ) amino acids , hydrophobic amino acids and polar amino acids of the protein were 13 . 74 % , 11 . 64 % , 36 . 45 % and 22 . 70 % respectively , and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site , protein kinase c phosphorylation site , casein kinase ii phosphorylation site , n - myristoylation site , camp - and cgmp - dependent protein kinase phosphorylation site , tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450 . a - helix and b - sheet of the protein is 47 . 7 % , 45 . 0 % respectively
Huangya14 )为材料分离克隆到一个细胞色素p450基因，命名为bccyp86mf5 ， cdna全长1854bp ，含1575bp的完整开放阅读框，编码524个氨基酸，其编码蛋白质的分子量为61 . 2kda 、等电点为8 . 96 ；碱性氨基酸、酸性氨基酸、疏水氨基酸和极性氨基酸分别占总氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二级结构预测包括n -糖基化位点、依赖于camp和cgmp的蛋白激酶磷酸化位点、蛋白激酶c磷酸化位点、酪蛋白激酶磷酸化位点、酪氨基酸激酶磷酸化位点、 n -豆蔻酰化位点和细胞色素p450的典型区域，半胱氨酸亚铁血红素配体信号区等， -螺旋和-折叠分别占47 . 7 、 45 . 0 ；与bccyp86mf1基因的氨基酸序列同源性达到95 . 2 ，与拟南芥cyp86c4的达到85 . 9 。